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click chemistry reaction  (Lumiprobe)


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    Structured Review

    Lumiprobe click chemistry reaction
    Click Chemistry Reaction, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/click+chemistry+reaction/Click+chemistry+buffer/bio_rxiv__2025__07__10__662049-183-1-15
    Average 93 stars, based on 14 article reviews
    click chemistry reaction - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Purification:

    Article Title: In vitro reconstitution reveals substrate selectivity of protein S-acyltransferases.
    Article Snippet: .. Consequently, the cells were lysed in 200 l of detergent-containing buffer (as described in the purification procedure) followed by performing click-chemistry reaction (using standard protocol) containing TAMRA azide (Lumiprobe), Tris[(1benzyl-1H- 1,2,3-triazol-4-yl)methyl]amine (TBTA), freshly prepared CuSO4, and TCEP, which were then added to the lysis supernatant and incubated for 1 h at 30 °C with intermittent mixing under dark condition. ..

    Article Title: In vitro reconstitution reveals substrate selectivity of protein S-acyltransferases
    Article Snippet: .. Consequently, the cells were lysed in 200 μl of detergent-containing buffer (as described in the purification procedure) followed by performing click-chemistry reaction (using standard protocol) containing TAMRA azide (Lumiprobe), Tris[(1-benzyl-1H- 1,2,3-triazol-4-yl)methyl]amine, freshly prepared CuSO4, and TCEP, which were then added to the lysis supernatant and incubated for 1 h at 30 °C with intermittent mixing under dark condition. ..

    Lysis:

    Article Title: In vitro reconstitution reveals substrate selectivity of protein S-acyltransferases.
    Article Snippet: .. Consequently, the cells were lysed in 200 l of detergent-containing buffer (as described in the purification procedure) followed by performing click-chemistry reaction (using standard protocol) containing TAMRA azide (Lumiprobe), Tris[(1benzyl-1H- 1,2,3-triazol-4-yl)methyl]amine (TBTA), freshly prepared CuSO4, and TCEP, which were then added to the lysis supernatant and incubated for 1 h at 30 °C with intermittent mixing under dark condition. ..

    Article Title: In vitro reconstitution reveals substrate selectivity of protein S-acyltransferases
    Article Snippet: .. Consequently, the cells were lysed in 200 μl of detergent-containing buffer (as described in the purification procedure) followed by performing click-chemistry reaction (using standard protocol) containing TAMRA azide (Lumiprobe), Tris[(1-benzyl-1H- 1,2,3-triazol-4-yl)methyl]amine, freshly prepared CuSO4, and TCEP, which were then added to the lysis supernatant and incubated for 1 h at 30 °C with intermittent mixing under dark condition. ..

    Incubation:

    Article Title: In vitro reconstitution reveals substrate selectivity of protein S-acyltransferases.
    Article Snippet: .. Consequently, the cells were lysed in 200 l of detergent-containing buffer (as described in the purification procedure) followed by performing click-chemistry reaction (using standard protocol) containing TAMRA azide (Lumiprobe), Tris[(1benzyl-1H- 1,2,3-triazol-4-yl)methyl]amine (TBTA), freshly prepared CuSO4, and TCEP, which were then added to the lysis supernatant and incubated for 1 h at 30 °C with intermittent mixing under dark condition. ..

    Article Title: In vitro reconstitution reveals substrate selectivity of protein S-acyltransferases
    Article Snippet: .. Consequently, the cells were lysed in 200 μl of detergent-containing buffer (as described in the purification procedure) followed by performing click-chemistry reaction (using standard protocol) containing TAMRA azide (Lumiprobe), Tris[(1-benzyl-1H- 1,2,3-triazol-4-yl)methyl]amine, freshly prepared CuSO4, and TCEP, which were then added to the lysis supernatant and incubated for 1 h at 30 °C with intermittent mixing under dark condition. ..

    other:

    Article Title: Choreography of human mitochondrial leaderless mRNA translation initiation
    Article Snippet: The click chemistry reaction was performed by incubating the clarified lysate with 30 μM Cy5-azide (Lumiprobe, #A3330), a premixed solution of 9.1 mM CuSO 4 (Fisher Scientific #AA1417836) and 45.45 mM THPTA (Vector Laboratories #CCT1010100), 100 mM sodium ascorbate (Fisher Scientific #AAA1775922), and 100 mM sodium phosphate buffer at 25 °C for 60 minutes.



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